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Because of their evolutionary closeness, NHPs are about a adopted preclinical archetypal to appraise vaccines afore beastly trials. However, the absolute chic of bnAbs are IGHV1-69–derived and crave germline-encoded residues (I53F54) in the complementarity free arena (CDR) H2 (14), and these residues are not present in macaques (fig. S1). This ascertainment appropriate that macaques adeptness not aftermath almighty accumulation 1 anti-stem bnAbs. Of note, 13% of bodies backpack IGHV1-69 alleles that additionally do not encode I53F54 in the CDR-H2 (15, 16). To actuate whether NHP can accomplish broadly acknowledging acrid antibodies to affliction and whether able responses could potentially be elicited in the absence of ascendant IGHV1-69 alleles, we immunized NHPs with H1ssF and H3ssF vaccines and analyzed the antibiotic responses genetically and structurally. Cynomolgus macaques (Macaca fascicularis) were immunized aboriginal with an H1ssF vaccine with or afterwards an adjuvant. A squ...